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• Yeast pheromone response model
  [[Fus1]] <br>
  18 KB (2273 words) - 19:08, 10 October 2008
• Pheromone/Receptor/G protein interactions
  ... mone in a [[ste2]]&Delta; strain. This was judged by halo formation and [[Fus1]]-lacZ expression. Chinault et al. 2004 PMID 14764600
  20 KB (2916 words) - 22:22, 24 July 2007
• Gpa1
  ... [[Gpa1]]), the GTPase-deficient [[Gpa1]](Q323L) mutant greatly increases [[Fus1]]-lacZ gene expression both in the absence and presence of [[pheromone]]. ...
  7 KB (930 words) - 21:11, 3 April 2008
• Ste11
  ... o alanine results in a non-functioning pathway (as judged by halo assay, [[FUS1]]-lacZ transcriptional reporter, and mating assay) ... o alanine) was able to function in the pathway (as judged by halo assay, [[FUS1]]-lacZ transcriptional reporter, and mating assay)
  4 KB (541 words) - 18:24, 1 November 2007
• Ste5/MAPK cascade interactions
  ... ensus binding sites has a moderate effect on pathway output (measured by [[Fus1]]-GFP, [[Fus3]] phosphorylation and mating efficiency), whereas simultaneo ... ... 7A p310A N315A) causes a 2-fold increase in pathway output (as judged by [[Fus1]]-GFP expression), suggesting that [[Ste5]] binding to [[Fus3]] may actual ...
  20 KB (3008 words) - 21:44, 5 January 2008
• Ste7/MAPK interactions
  ... [[Ste5]] V763A S861P) activates the same amount of transcription off the [[FUS1]] gene as a [[Ste5]]-null mutant. Bardwell et al. 2001 PMID 11134045 ... ensus binding sites had a moderate effect on pathway output (measured by [[Fus1]]-GFP), whereas simultaneous mutation of both MAPK consensus binding sites ...
  9 KB (1329 words) - 20:24, 5 January 2008
• Dig2
  ... ) and on mating response (judged by growth arrest ([[BAR1]] strain), and [[Fus1]] gene expression). ... s), although exposure to pheromone still results in a modest increase in [[Fus1]] gene expression.
  2 KB (273 words) - 21:34, 26 September 2006
• MAPK phosphorylation cascade
  ... o alanine results in a non-functioning pathway (as judged by halo assay, [[FUS1]]-lacZ transcriptional reporter, and mating assay). ... o alanine) was able to function in the pathway (as judged by halo assay, [[FUS1]]-lacZ transcriptional reporter, and mating assay).
  34 KB (5052 words) - 20:29, 23 October 2008
• Dig1
  ... ) and on mating response (judged by growth arrest ([[BAR1]] strain), and [[Fus1]] gene expression). ... s), although exposure to pheromone still results in a modest increase in [[Fus1]] gene expression.
  2 KB (327 words) - 21:35, 26 September 2006
• Dig1/Dig2/Ste12 phosphorylation
  ... '' cells, treatment with [[pheromone]] does not affect the expression of [[FUS1]]-''LacZ''. Bardwell et al. 1998 PMID 9860980; Tedford et al. 1997 PMID 9 ... ... one]], or phosphorylation of [[Ste12]] does not affect the expression of [[FUS1]]-''LacZ''.
  14 KB (2013 words) - 22:45, 24 July 2007
• Ste12
  ... of the N-terminal 20 amino acids of [[Ste12]] results in 30-fold higher [[FUS1]]-''LacZ'' expression in the absence of [[pheromone]]. Crosby et al. 2000 ...
  7 KB (1026 words) - 21:27, 4 September 2007
• Ste11/Ste50 interactions
  ... sult in sustained cell cycle arrest (by halo assay), or gene expression ([[FUS1]]-LacZ reporter).
  7 KB (1043 words) - 21:40, 20 March 2007
• Ste4:Ste18/Ste5 interactions and Ste5 dimerization/oligomerization
  ... he absence (40-fold) and presence (2-fold) of [[pheromone]] (measured by [[Fus1]]-LacZ reporter) **''[[ste4]]&Delta;'' [[STE5]]-GST strain has the same [[Fus1]]-LacZ levels in the absence of [[pheromone]] as WT cells. This suggests ...
  21 KB (3081 words) - 14:26, 21 October 2008
• Cell cycle regulation of pheromone response
  **Deletion of CLN1 and CLN2 eliminates cell cycle repression of [[FUS1]] expression. **Overexpression of CLN1 or CLN2 represses [[pheromone]]-dependent [[FUS1]] expression.
  6 KB (834 words) - 19:02, 24 September 2007
• Dig1/Dig2/Ste12/Tec1 interactions
  ... on domain and [[Dig1]] or [[Dig2]] results in constitutive expression of [[FUS1]]-''lacZ'', as well as a constitutive invasive growth phenotype. Tedford ... **[[Ste12]](Y310A Y317A) has 6x higher [[FUS1]]-''lacZ'' expression than WT [[Ste12]] in the absence of pheromone, but t ...
  31 KB (4382 words) - 22:45, 24 July 2007
• Mutants for fitting model
  ... [[Ste5]], [[Fus3]], [[Kss1]], and [[Fus3]] and [[Kss1]] on expression of [[FUS1]]-''LacZ'' in figure 5 of Crosby et al. 2000 PMID 11054817 *Effect of [[Kss1]] deletion or non-phosphorylatable [[Kss1]] on [[Fus1]]-''LacZ'' expression at several time points in figure 3 of Sabbagh et al. ...
  2 KB (230 words) - 19:15, 20 July 2007

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