Ste2 synthesis/endocytosis/degradation

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Contents 1 Ste2 Synthesis 1.1 Reaction Definition 2 Ste2 Internalization/Degradations 2.1 Reaction Definition 3 Ste3 Internalization/Degradations 3.1 Reaction Definition

Ste2 Synthesis

• In the absence of pheromone constitutive internalization of Ste2 has a half-time of ~45 minutes. Hicke et al. 1998 PMID 9548714
  • This gives an internalization rate of ln(2)/45min ≅ 2.6 * 10^-4 s^-1.

• Ste2 synthesis rate of 4 molecules per cell s^-1 was measured or inferred. Yi et al. 2003 PMID 12960402
  • This same value was also subsequently used (referenced to Yi et al. 2003) by Kofahl and Klipp 2004 PMID 15300679
  • It is assumed that the steady state internalization rate and number of Ste2 molecules per cell were measured (probably via a Scatchard plot), and the synthesis rate inferred.
  • The steady state internalization rate of 4 * 10^-4 s^-1 was used. Yi et al. 2003 PMID 12960402

Reaction Definition

Synthesis of Ste2 must balance the constitutive internalization of Ste2 in the absence of pheromone, to give the steady state amount of Ste2 (Ste2_tot_conc). Since th