Yck

From Yeast Pheromone Model

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Contents 1 General Comments 2 Yck1 3 Yck2 4 Yck3 5 Reactions 6 Species Representation 6.1 Molecule Type 6.2 Model Seed

General Comments

• Yck1, Yck2 and Yck3 are serine/threonine protein kinase
  
• Part of the Casein Kinase I (CKI) family. Wu et al. 2003 PMID 14555477
• All three Yck proteins have a similar specific activity on casein. Sternbach and Kuntzel 1987 PMID 3311146
• Yck1 and Yck2 proteins are required for the phosphorylation, ubiquitinylation and internalization of Ste2. Hicke et al. 1998 PMID 9548714
• The canonical CKI target consensus sequence is (D/E)_1-3-X-X-S/T. Wu et al. 2003 PMID 14555477

Yck1

• Potential palmitoylation sites at C537 and C538. Swiss-Prot
• Yck1 strongly prefers ATP as phosphate source. Sternbach and Kuntzel 1987 PMID 3311146

Yck2

• Palmitoylated at C545 and C546 by Akr1(required for membrane localization). Roth et al. 2002 PMID 12370247
  
• [Yck2]] strongly prefers ATP as phosphate source. Sternbach and Kuntzel 1987 PMID 3311146

Yck3

• Potential geranyl-geranylation sites at C523 and C524. Swiss-Prot
  
• Yck3 is able to use both ATP and GTP efficiently as phosphate sources. Sternbach and Kuntzel 1987 PMID 3311146
• Yck3 has a kcat = 2.4 /s for casein target (Sternbach et al., Biochem (26) 4207)
  
• Appears not to be important for adaptation in pheromone response. Hicke et al. 1998 PMID 9548714

Reactions

Receptor(Ste2) synthesis/endocytosis/degradation
Protein dilution/synthesis due to cell growth

Species Representation

Molecule Type

Yck(Ste2_site)

Model Seed

Yck(Ste2_site) Yck_tot_conc